Tissue Sample Collection is Strictly Confidential!
Blood
or tissue samples are generally required for gene hunting projects. If a cat
must be euthanized or has died, tissue samples or the entire cadaver can be
collected for DNA isolation. If tissue samples can not be collected immediately
by a veterinarian, the cadaver should be frozen. DNA degrades rapidly in tissues,
thus samples should be sent frozen immediately was procured from the individual.
Tissues samples that degrade less slowly include spleen and kidney and are
the preferred tissues. The liver has many degrading enzymes and glycogen stores,
thus liver is NOT a preferred tissue. Send any portions of the tissue that
are not required for necropsy. Formalin fixed tissues are extremely difficult
for DNA isolation and destroys the DNA into small pieces. Please do not put
the samples for DNA analysis in formalin. Formalin fixed tissues may be required
for the pathology of specific projects (see Formalin fixed tissues).
Tumors should be sent
immediately, but on ice, NOT frozen! Extremely important, please plan ahead
if possible! We will attempt to grow the cells from the tumor, freezing will
kill the cells. Insulate the tumor from the ice packs to prevent freezing.
Wrap the tumor in a paper towels that are saturated with saline then place
in a baggie.
Instructions
for sending tissue samples:
Samples need to be sent
by overnight courier, thus please arrange for the samples to be collected
early in the week.
When you make the veterinary
appointment, please inquire if the sample can be picked-up by an overnight
courier from the clinic, otherwise you must be prepared to get the sample
to the courier.
You must package the
sample as described below, thus be prepared with the packaging.
A FEDEX account number can
be used for funded projects, thus please contact the lab for the account number.
Please send an e-mail to the lab The
Lyons' Den or call: (530) 752-7127.
Freeze the tissue sample until
shipping the next day.
If a sample can not be sent
for several days, the sample should remain frozen until shipping.
If the sample must
be sent on a Friday, please contact the lab for a different address for a
Saturday delivery. Please send an e-mail to the lab The
Lyons' Den or call: (530) 752-7127.
Notify the lab that
the sample is being shipped. Please send an e-mail to the lab The
Lyons' Den or call: (530) 752-7127. Please provide your contact information,
especially your own phone and or e-mail address, so that we can notify you
of the sample's arrival.
Please include details on
the sample, such as a pedigree and project information. Many samples come
to the lab and may be processed by various individuals.
Packaging Instructions:
Place the tube/tissue/ cadaver
in a zip lock baggie. Label with the type of tissue if appropriate.
Place the sample in a small
box with many frozen ice packs.
The sample should stay frozen,
thus be sure to include at least two ice packs.
Many projects have
special funding thus the cost for the collection of samples for these projects
can be re-imbursed. Re-imbursement can be made directly to the owner or the
veterinary clinic. The owner will have to provide a proper mailing address,
phine number and social security number to be established as a vendor by the
university. Re-imbursement should take only a week or so. Re-imbursement can
also be made directly to the veterniary clinic if they will accept a payment
after the procedure. Invoices should be sent with the samples or by a separate
mailing.
All samples are for
research purposes only. Any donations of sampling costs and shipment expenses
is greatly appreciated!
Last update: November 30, 2004 This site is under continual
development. Please visit again soon! Many of the current pages are the place
holders for future information. Please provide suggestions to the webmaster:
felinegenome@ucdavis.edu
