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Boar sperm double-stained using FITC-PSA (lectin) and ethidium homodimer. (left)
Equine sperm double-stained using FITC-PSA and ethidium homodimer. (right) |
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Confocal double imuunofluorescence of macaque sperm labeled using Cy5-conjugated polyclonal rabbit anti-human caveolin-1 (green fluorescence) and the lipophilic fluorescent dye, DiI (1,1’-dioctadecyl-3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate) to label sperm membrane rafts. a) control, freshly ejaculated washed sperm, 22oC, (60X oil immersion); b) 80% percoll-washed sperm, 22oC, (60X oil immersion); c) in vitro capacitated sperm, activated with 1 mM dbcAMP + caffeine, 22oC, (100X oil immersion). | |||||||||
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Representative macaque sperm double-labeled with Mitotracker® (MITO) and PI. (A) Live sperm showing high fluorescence intensity MITO and no PI staining indicating high mitochondrial membrane potential (MMP). (B) Non-viable sperm (PI+ head) showing moderate to low MMP. (C) Non-viable sperm (PI+ head) showing no MITO staining, very low MMP. | |||||||||
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Three normal equine sperm, differential interference contrast (Nomarski optics), 100X oil. | |||||||||
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Tyrosine phosphorylation immunofluorescence patterns of equine sperm.Cells were incubated in BWW medium at 37C in humidified 95% air and 5% CO2 atmosphere for 3 h. Four different patterns were observed: (a) equatorial band, (b) tail, (c) equatorial band with tail, and (d) none. Scale bar 5.5 mm. |
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Equine sperm viability as detected by SYBR-14 and propidium iodide (Molecular Probes, Inc). green cells are viable, red cells are non-viable. | |||||||||
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