Atwill Water & Foodborne Zoonotic Disease Laboratory

Ronald F. Bond

Photo: Bond


1993-1995 - AA
Mississippi Gulf Coast Community College
Major: Environmental Policy

2010-2012 - B.S.
University of California, Davis, CA
Major: Community and Regional Development


I am interested in studying the correlation of indicator bacteria on freshwater systems and the ways these bacteria signify the presence of pathogenic bacteria. I have been interested in new methods of looking at agricultural systems as point sources and ways to reduce the risk of pathogen transport out of these systems.


  • Reducing agricultural pathogens into the Sacramento-San Joaquin Delta estuary
  • Using scent detection dogs to prioritize samples during produce outbreak traceback investigations
  • Epidemiologic analysis and risk management practices for reducing E. coli in irrigation source water supplies and distribution systems
  • Efficacy of NRCS approved sediment basins used in agricultural settings


  • Design of efficient methods of membrane filtration of E-coli, Enterococcus, and fecal coliforms.
  • Designed sampling protocols for studying ambient freshwater systems.
  • Responsible for collection of water samples using drop samplers, YSI mulitprob sondes, flow meters, GPS enabled depth meter, and various other meteorological equipment.
  • Use of IMS technique for quantification of Cryptospiridium and Girardia.
  • Help design protocols for analysis of water and soil samples.
  • Oversee student and lab helper positions and scheduling work hours, along with supervisor.
  • Biochemical analysis of E. coli isolates, including Gram staining.
  • Help graduate student with methods for detecting E. coli, Enteroccus, Salmonella, Camplyobacter and fecal coliforms in water and soil.
  • Collect water samples for preliminary testing following USEPA/FDA protocols.
  • Lead field and laboratory operations for the Mississippi Beach Monitoring Program. Field collection and analysis of samples using MPN and membrane filtration procedures were performed on water, sediment, and oyster meat samples.
  • Field collection of water samples and general laboratory assistance provided during Mississippi’s Coastal Watersheds TMDL Project (Bay St. Louis). Laboratory assistance provided for total dissolved solids, BOD, fecal coliform enumeration, and Chlorophyll a analyses.
  • Maintained a database for watershed biotic and abiotic parameters (e.g., temperature, dissolved oxygen, salinity, pH, alkalinity, hardness, conductivity, etc.)
  • Coordinated sample collections from sewerage lift stations and analyzed effluent discharge water samples in support of grants and contracts focused on monitoring human health risks of bathing beaches and residential areas.
  • Supervised and trained graduate student laboratory workers in media preparation, decontamination of waste, general laboratory sanitary practices, and light field work and sample collection.
  • EPA Beach Watch Protocol for point source and non-point source pollution.
  • Fulfilled requirements for FDA Split Sample Program for Interstate Shellfish Sanitation Conference. This was performed annually. Other annual proficiency tests were performed as required by management and/or contractor requirements under HACCP.
  • Identified gram-negative organisms on API 20Ne and IMViC, gram staining, and acid fasting.
  • Enterococcus, E coli, and fecal coliform enumeration using the current EPA methods 1103.1, 1106.1, 1600, 1603, 1680, 1681 and other wastewater methods stated in Standard Methods.
  • Effectively maintained QA/QC laboratory programs for the Mississippi Shellfish Program, The Mississippi Beach Monitoring Prrogram, Mississippi Bay of St. Louis Watershed TMDL Wet Weather Project, Coastal Impact Assistance Program grants for Bacterial Source Tracking, 303(d) and 305(b) analytical support for Mississippi Department of Environmental Quality EPA water monitoring programs, previously known as Ambient Water Monitoring Program, and any other grant or project conducted during employment period.


  • 2008. Lewis, D.J., E.R. Atwill, M.D.G. Pereira, R.F. Bond, M. Huerta. Estuary sediment and suspended solids bacteria: implications for microbial pollution sources and monitoring.2008 USDA-CSREES National Water Conference. Sparks, NV. February 3-7.
  • 2009. Atwill Edward R.,Melissa L. Partyka, R.F. Bond, Xunde Xi,“An Introduction to Waterborne Pathogens in Agricultural Watersheds.” Technical Report. NRCS.
  • 2010. Bond, R.F., M.L. Partyka, E.R. Atwill, "THE OCCURRENCE OF INDICATOR BACTERIA AND WATERBORNE ZOONOTIC PATHOGENS IN THE CALIFORNIA DELTA" Presented at 6th Biannual Bay-Delta Science Conference,Tuesday, Sacramento, Ca, September 28, 2010.