Center for Children's Environmental Health

Core 3 - Analytical Chemistry

The overall objective of the Analytical Chemistry Core is to provide analytical support for the human monitoring, animal model, and cell biology projects in the Children's Center. We apply techniques to the research projects as appropriate, and advance the technology upon which the analytical methodology is based. This core conducts new analyses on serum samples from the CHARGE study, CHARGE-BACK and MARBLES studies. We evaluate new methods and test specific hypotheses. A major goal is to generate data sets of xenobiotic exposure and metabolomic biomarkers to regress against transcriptome and proteome data by determining both xenobiotic exposure and levels of endogenous metabolites on selected. We evaluate metabolite profiles in animal models emphasizing immune dysfunction. In human samples, we pay particular attention to metabolites indicative of inflammatory status and plasma leptin levels recently found as a biomarker to distinguish between early onset and clinical regression autism. We also monitor the metabolite profiles in T cells, B cells and natural killer (NK) cells of normal and autistic children with and without exposure to xenobiotics. Of equal importance we provide a walk up instrument and consultation service in analytical chemistry.  Our Specific Aims are to: 1. Establish the metabolic pathways responsible for variations in lipid composition between autistic children and their siblings and between normal and immunologically challenged animal models of autism. 2. Use global metabolomic procedures to search for biomarkers of autism. 3. Provide analytical data on pesticide and other xenobiotic levels in cell and in vivo model systems and in serum samples from the CHARGE, CHARGE-BACK, MARBLES, and other successor studies. 4. Develop new analytical methods for xenobiotics of interest to scientists in the program project.


Core Leader

Bruce D. Hammock, Ph.D.
Department of Entomology 

Co-Leaders:

Bruce German, Ph.D.
Department of Food Science

Peter Green, Ph.D.
Department of Civil and Environmental Engineering

Jun Yang, Ph.D.
Department of Entomology