About 100 ml of this solution is adequate for one large eye or several small eyes. The eye should be placed in the solution immediately after enucleation and trimming. A gauze pad should be used to keep the eye submerged if necessary or inconsistent fixation will occur. The globe should remain in the solution for one to two days. The eye then is taken out of the solution and placed either in formalin or 70% ethanol. To make the eye easier to cut, you could leave it in 70% ethanol for a day, transfer to 90% for a day and finally to 100% for a day.
Be sure to dispose of the original Davidson's solution and other contaminated fluids appropriately.