Optic nerve cross section

Source 7 month male Borzoi, OD
Fixative Zenker's
Stain Bodian
Notes Normal tissue; each tiny black dot or line within each fascicle is an axon cylinder which has picked up the silver in the Bodian stain (not every cylinder gets stained, which is one of the vagaries of this method)
See also azure II-methylene blue
Luxol fast blue
Legend arrow - points to extensions of pia mater which form the septae (along with astrocytes) that surround each fascicle, F - fascicle
Illustration Click on image to see larger picture