Equine Viral Disease Laboratory
UC Davis School of Veterinary Medicine
 
ceh photo
 
 

The laboratory has a truly outstanding scientific staff with the technical expertise necessary to maintain a state-of-the-art molecular virology facility dedicated to the study of viral diseases of the horse. Two primary objectives are:

bullet graphic Maintain the diagnostic capability and expertise of the laboratory through strategic partnering with pre-eminent groups throughout the United States and the world. These collaborations have rapidly expanded the laboratory’s capability so that the scientific staff have become facile in characterizing endemic diseases such as equine herpes, West Nile and equine viral arteritis, and diseases that currently are exotic to the United States such as African horse sickness.
   
Continue cutting edge research on viral diseases of the horse that is important to the regional industry, diseases such as equine viral arteritis. These studies are especially focused on the development of new diagnostic and vaccine technologies, definition of the epidemiology and pathogenesis of important viral diseases of the horse, and the recognition of new and emerging viral diseases of the horse. These studies incorporate state-of-the-art research technologies.

Our laboratory has research expertise in the following areas:

Classical virologic techniques and procedures
Virus isolation
  Serology, including serum neutralization, ELISA, western immunoblotting
     
Molecular virologic and diagnostic techniques
  Polymerase chain reaction (PCR)
  Reverse transcriptase PCR (RT-PCR)
    Nested PCR
    Real-time PCR
  Southern and northern blotting
  Nucleic acid sequencing
  Genome manipulation procedures such as infectious cDNA clones
  Modern strategies for nonreplicating vaccine development
    Subunit vaccines
    DNA vaccines
    Novel “targeted” vaccine delivery systems
  Production and characterization of monoclonal antibodies
     
Epidemiology and molecular epidemiology
  Genetic characterization of microbial evolution
  Genetic determinants of virus persistence and virulence
   
Pathology and pathogenesis
  Necropsy and histopathology
  Immunohistochemistry
  In situ hybridization
  Electron microscopy
  Flow cytometry
  Microarry