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UC Davis Veterinary Medicine Extension
Veterinary Medicine Teaching and Research Center


Sterile Milk Sampling

John H. Kirk, DVM, MPVM

Veterinary Medicine Extension
University of California, Davis

Sterile infusion techniques must be used in collecting milk samples when culturing for microorganisms that cause mastitis. Non-sterile collection of milk leads to errors in diagnosis of the causes of mastitis and a waste of time and money. Using the f ollowing suggested steps to avoid contamination samples when collecting milk samples for culture.

Suggested supplies:

 

 

Collect the sample after milking.

 

 

Clean teat ends until the cotton or pad comes off teats white.

Begin with teats farthest away to avoid touching them while working on remaining teats.

 

 

This avoids bacteria from the streak canal…want samples from the gland.

 

 

Don't place cap on floor or curb as it might become contaminated.

 

 

 

 

Fresh milk samples should be delivered to the laboratory within 12 hours after collection.

Samples can be frozen for later shipment to the laboratory after the outcome of any treatment is known or for periodic mastitis monitoring.

 

 

Consult your local veterinarian for information on interpretation of the culture results.

 

Milk samples may be sent to:

Milk Quality Laboratory
Veterinary Medical Teaching and Research Center
University of California, Davis
18830 Road 112
Tulare, CA 93274

 

 

Information and pictures were taken from:

Warren D. Gilson, Extension Dairy Scientist

James D. Strawser, Extension Editor

University of Georgia

College of Agricultural and Environmental Sciences

Sponsored by: Pharmacia & Upjohn


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