UC Davis School of Veterinary Medicine

School of Veterinary Medicine



 

LEPTOSPIROSIS SAMPLE SUBMISSION FORM Shipping address, payment, and shipping instructions are included on this form.


What we offer for leptospirosis diagnostics:

1. Real-time PCR for the detection of Leptospira spp. DNA. Samples that can be used include urine, CSF, aqueous humor or whole blood (LTT). ($42.00 for one sample)
When to run PCR- if you suspect animal is actively shedding, case is early (before antibodies would be detectable), or you are attempting to diagnose active infection. We recommend you submit blood and urine for live animals or kidney tissue if animal has died.
How to submit a sample for PCR- Keep samples cold (refrigerator/ice pack) at all times. Use appropriate biohazardous material handling. Submit 2 ml whole blood in EDTA (lavender top) tube, 5-10 ml urine in EDTA (lavender top) tube or red-top tube or other leak proof container, or a small (5 g) piece of frozen tissue in a ziplock bag or leak-proof container. Aspirate in a syringe is acceptable. Other samples such as aqueous chamber fluid or cerebral spinal fluid are acceptable in a red-top tube as well.

2. Serologic detection using microscopic agglutination testing (MAT). Serum will be screened against a panel of seven serovars (L. canicola, L. hardjo, L. icterohaemorrhagiae, L. pomona, L. bratislava, L. mini, and L. grippotyphosa). Initial and convalescent samples are strongly recommended. (Timing of convalescent sample will be determined after results from the initial sample are known.) Serum or whole blood can be tested. ($50.00, initial and convalescent: $75.00 total for both)
When to run serology- Serology is appropriate to confirm vaccine titers, assess acute and/or convalescent titers for production of antibodies to support a clinical diagnosis, and to determine which serovar the animal has been exposed to.
How to submit a sample for serology- Keep samples cool or frozen. Submit at least 0.5ml blood in a red top or serum separator tube. Whole blood collected in an EDTA tube can also be used.

3. Culture from samples (urine, blood, CSF, aqueous humor, kidney) collected prior to initiation of antibiotic treatment. ($50.00)
When to run culture- To confirm active infection, to obtain isolates for downstream characterization including virulence, molecular characterization, etc.
How to submit a sample for culture- For culture samples contact the UC Davis Leptospirosis Diagnostic Laboratory at 530-754-9740 for more information regarding sample collection and shipping requirements. At least 0.5 ml fluid or tissue should be collected, kept cool (not frozen) and shipped overnight. CULTURE CANNOT BE RUN AFTER ANTIBIOTICS (ANY DOSAGE, EVEN A SINGLE TIME) HAVE BEEN GIVEN.

    Sample collection information: Keep samples cold (refrigerator/ice pack) at all times.


    Serum – For serology: at least 0.5ml in a red top or serum separator tube. Whole blood collected in an EDTA tube can also be used.  

    Whole blood – For Real-time PCR: 2 ml in EDTA (lavender top) tube.
    - For culture: contact the UC Davis Leptospirosis Diagnostic Laboratory at 530-754-9740 (ask for Janet Foley or Mary Straub).

    Urine - For Real-time PCR: ~5-10 ml in EDTA (lavender top) tube or other leak proof container.
    - For culture: contact the UC Davis Leptospirosis Diagnostic Laboratory at 530-754-9740 (ask for Janet Foley or Mary Straub).

    Cerebral Spinal Fluid (CSF) - For Real-time PCR: at least 0.5ml in red or lavender top tube. Sample should have been drawn within the past three days.
    - For culture: at least 0.5ml collected aseptically into a red top (no additive) tube, kept cold and shipped overnight.

    Aqueous fluid – For Real-time PCR: at least 0.5 ml in EDTA (lavender top) tube.
    - For culture: any volume collected aseptically into a red top (no additive) tube or other sterile container (aspirate in a syringe is acceptable), kept cold and shipped overnight.

    Kidney – For Real-time PCR - 25-100 mg of fresh or frozen tissue with no additive (aspirate in a syringe is acceptable), kept cold and shipped overnight.
    - For culture: 25-100 mg of fresh tissue collected aseptically into a red top (no additive) tube, or other sterile container (aspirate in a syringe is acceptable), kept cold and shipped overnight.