Emerging Disease – Canine Influenza A H3N2 Virus
In April 2015, an outbreak of influenza A virus occurred in the Midwest affecting nearly 1,000 dogs. The strain responsible for disease was H3N2, a novel strain to the United States, but previously reported in Asia in 2006/2007. The Core Facility currently offers an influenza A H3N8 virus real-time PCR test, however this assay will not detect H3N2. A new H3N2 assay is in the process of being validated and expected to be offered to the public as a standalone test or as part of the Canine Respiratory Panel as early as mid-June 2015.
For more information on influenza A H3N2 virus please visit:
Pusterla et al., 2013. Emerging outbreaks associated with equine coronavirus in adult horses. Veterinary Microbiology. Vol 162. pp. 228-231
Don’t know much about equine coronavirus (ECoV)? You’re not alone! Recent research from 2011 and 2012, however, has shed some light on this little known virus. Patients infected with ECoV commonly have unspecific clinical signs such as fever, lethargy, and anorexia. Less commonly, colic and changes in fecal character can occur. Although generally self-limiting, secondary infections due to gastrointestinal translocation can take place causing more severe complications, even death. Currently, molecular detection of the virus in feces is the only diagnostic method available. At the Real-time PCR Core Facility we offer ECoV as a standalone test, and as part of a comprehensive Equine GI/Diarrhea Panel (ECoV, Lawsonia intracellularis, Potomac Horse Fever, Salmonella spp, and Clostridium difficile toxins A and B). Results are relayed quickly, which means you can make a diagnosis and start treatment promptly. Because ECoV is contagious, rapid diagnosis means proper biosecurity measures can be implemented to prevent disease spread. In addition, our board-certified veterinarians are available for assistance in PCR interpretation. Download our submission packet for pricing and sample information!
Pusterla et al., 2010. Use of quantitative real-time PCR for the detection of Salmonella spp. in fecal samples from horses at a veterinary teaching hospital. Vet J Nov;186(2):252-5
Pusterla et al., 2014. Investigation of the use of pooled faecal and environmental samples following an enrichment step for the detection of Salmonella enterica by real-time PCR. Vet Rec;Feb25.
We are currently implementing a selenite broth enrichment protocol for all samples submitted for Salmonella spp testing. PCR testing for Salmonella post culture greatly increases detection rates compared to testing on fresh feces alone. Please refer to our brochure for more information.
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