LEPTOSPIROSIS SAMPLE SUBMISSION FORM
What we offer for leptospirosis diagnostics:
Real-time PCR for the detection of Leptospira spp. DNA. Samples that can be used include urine, CSF, aqueous humor or whole blood (LTT). ($42.00)
Serologic detection using microscopic agglutination testing (MAT). Serum will be screened against a panel of seven serovars (L.canicola, L.hardjo, L. icterohaemorrhagiae, L.pomona, L.bratislava, L.mini, and L.grippotyphosa). Initial and convalescent samples are strongly recommended. (Timing of convalescent sample will be determined after results from the initial sample are known.) Serum or whole blood can be tested. ($50.00, initial and convalescent: $75.00 total for both)
Culture from samples (urine, blood, CSF, aqueous humor, kidney) collected prior to initiation of antibiotic treatment. Culture is not indicated if any antibiotics have been administered prior to sample collection. For culture samples contact the UC Davis Leptospirosis Diagnostic Laboratory at 530-754-9740 (ask for Janet Foley or Mary Straub) for more information regarding sample collection and shipping requirements. ($50.00)
Sample collection information: Keep samples cold (refrigerator/ice pack) at all times.
Serum – For serology: at least 0.5ml in a red top or serum separator tube. Whole blood collected in an EDTA tube can also be used.
Whole blood – For Real-time PCR: 2 ml in EDTA (lavender top) tube.
- For culture: contact the UC Davis Leptospirosis Diagnostic Laboratory at 530-754-9740 (ask for Janet Foley or Mary Straub).
Urine - For Real-time PCR: ~5-10 ml in EDTA (lavender top) tube or other leak proof container.
- For culture: contact the UC Davis Leptospirosis Diagnostic Laboratory at 530-754-9740 (ask for Janet Foley or Mary Straub).
Cerebral Spinal Fluid (CSF) - For Real-time PCR: at least 0.5ml in red or lavender top tube. Sample should have been drawn within the past three days.
- For culture: at least 0.5ml collected aseptically into a red top (no additive) tube, kept cold and shipped overnight.
Aqueous fluid – For Real-time PCR: at least 0.5 ml in EDTA (lavender top) tube.
- For culture: any volume collected aseptically into a red top (no additive) tube or other sterile container (aspirate in a syringe is acceptable), kept cold and shipped overnight.
Kidney – For Real-time PCR - 25-100 mg of fresh or frozen tissue with no additive (aspirate in a syringe is acceptable), kept cold and shipped overnight.
- For culture: 25-100 mg of fresh tissue collected aseptically into a red top (no additive) tube, or other sterile container (aspirate in a syringe is acceptable), kept cold and shipped overnight.
