Real-time PCR Research and Diagnostics Plant Pathogen Testing Service Information

 

This service is currently available only for grapevines originating in California - samples from outside of California or the U.S. will not be accepted.

 

Available Panels

Initial Project Setup

Sample Collection

Shipping Instructions

Sample Submission Form

 

Available Panels

Panel A: $340
Grapevine leafroll associated virus 1, Grapevine leafroll associated virus 2, Grapevine leafroll associated virus 2 Red Globe, Grapevine leafroll associated virus 3, Grapevine leafroll associated virus 4, Grapevine leafroll associated virus 5, Grapevine leafroll associated virus 7, Grapevine leafroll associated virus 9, Grapevine leafroll associated virus 10, Grapevine leafroll associated virus Carnelian strain, Grapevine rupestris stempitting associated virus, Grapevine virus A, Grapevine virus B, Grapevine virus D, Grapevine virus E, Grapevine fleck virus.

Panel B: $200
Tobacco ringspot virus, Tomato ringspot virus, Grapevine fanleaf virus.

Xylella fastidiosa: $50
Possible add-on to Panel A or B (not available alone)

 

Initial Project Setup

1. Submit the completed request form to Cathy Inta & Tammi Olineka via e-mail or FAX (530-754-6862). Please check with us to ensure the request was received.  
2. A quote will be issued and mailed back along with the “Agreement for Provision of Plant PCR Testing Services” form.
3. Submit the following:
   • Signed "Agreement for Provision of Plant PCR Testing" form.
   • Exhibit 13A form
   • Completed submission form, or Excel format Sample Submission form (10+ samples)
   • If needed, e-mail large submission form to Cathy Inta
     

Sample Collection

Grapevine Pathogens (Panel A) starting October 1st

1. Submit six 10 inch cane cuttings at least 1cm in diameter per mature grapevine (defined as one sample)
   • Use gloves when collecting samples and change gloves between grapevines
   • Remove petioles (if desired) by pulling from the leaf
2. Wrap cuttings in a moist paper towel and place in a clearly labeled ziploc bag
3. Store samples at 4C until shipped. When collecting samples in the field, place them in a cooler with covered icepacks. Do not place samples directly on ice.

Grapevine Nepoviruses (Panel B) from early spring through June

1. Submit 15-20 petioles per grapevine
   • Use gloves when collecting samples and change gloves between grapevines
   • Remove petioles by pulling from the leaf - DO NOT CUT OR REMOVE LEAVES
2. Wrap petioles in a moist paper towel and place in a clearly labeled ziploc bag
3. Store samples at 4C until shipped. When collecting samples in the field, place them in a cooler with covered icepacks. Do not place samples directly on ice.

*Xylella fastidiosa can be added to either panel A or panel B

Shipping Intructions

1. Samples should be in a clearly labeled ziploc bag.
2. Include a completed Sample Submission Form with each shipment.
3. Samples should be shipped in a rigid container (i.e. cardboard or styrofoam box).
4. Include ice packs covered with absorbant material to absorb condensation and protect samples.
5. Ship overnight (the laboratory is open Monday-Friday) via FedEx or other express courier.
   • Do NOT use USPS as all packages are held at a central campus distribution center.

Sample Submission Form