UC Davis School of Veterinary Medicine

School of Veterinary Medicine


Real-time PCR Research and Diagnostics Core Facility accepts research projects from both academic and private sector. The Core Facility can accommodate projects involving RNA and DNA extractions through semi-automated nucleic acid extractions systems, cDNA synthesis and complete Real-time analysis using a laser 7900 HTA FAST platform. Real-time PCR Research and Diagnostics Core Facility provides research results efficiently and rapidly while adhering to the highest quality standards and offers a unique opportunity to concentrate the latest equipment and knowledge in one location.


Upcoming Real-time PCR Readiness Workshop


September 8th-11th, 2014 (lecture - 1102 Gourley)

September 9th-11th, 2014 (wet lab - 3403 Tupper Hall)

September 15th-17th, 2014 (second section of wet lab)


Download course schedule here

Need directions? An interactive campus map can be found here

Real-time Polymerase Chain Reaction is becoming increasingly more useful in many laboratories due to its continuing advancements. This course covers an in-depth view in the theoretical and practical knowledge of quantitative PCR (qPCR) techniques, and is intended for academic or industrial persons considering working with qPCR or scientists currently working with qPCR seeking a deeper understanding. Lectures are open to all participants, while a supplemental wet lab is offered at an additional cost on a first come first served basis.

Participants will learn about qPCR theoretical concepts, experimental design, primer/probe design, applications, sample preparation and extraction, quality controls of gDNA and total RNA, contamination control and monitoring for amplification reactions, optimization and troubleshooting of qPCR, gene expression, mRNA, siRNA, quantification strategies in RT-qPCR, and data analysis and interpretation.

Topics discussed in lecture include molecular diagnostics in single-cells, high throughput qPCR analysis, minimum information for publication of qPCR experiments (MIQE) guidelines, small RNAs, pre-analytical steps, and qPCR biostatistics and bioinformatics. The course will also examine the latest developments, opportunities and applications of both digital PCR and qPCR through case studies across diverse fields.

The wet lab offers extensive hands-on training by qPCR experts from the Real-time PCR Core Facility team. Leading probe technologies will be demonstrated, common and emerging instrument platforms will be available, and advanced primer and probe design programs will be utilized. Hands-on training will be provided in sample preparation, nucleic acid extraction, and quantitative gene expression, including design and optimization of both RT and qPCR protocols.

Lecture + Lab (student) = $345

Lecture + Lab (academic) = $460


Lecture only (student) = $125

Lecture only (academic) = $170

To register please call the Core Facility at 530-752-7991 or email Cara Wademan at carjohnson@ucdavis.edu