UC Davis School of Veterinary Medicine

School of Veterinary Medicine



Research Services

(scroll down for service price list)

Pre-analysis consulting. Consulting services are available including protocols, discussion of sample types, sample number, genes of interest and overall goals of the project.

Sample preparation. Assistance in sample preparation is available including protocols and provision of sample stabilization solution.

Extractions and cDNA synthesis. gDNA and total RNA extractions for research projects are done in a 96-well based format using Qiagen BioSprint. Additionally researchers can request to have their samples precipitated and cDNA synthesized. A housekeeping gene validation experiment is run prior to profiling in order to determine the most stably transcribed housekeeping gene.

Special protocols. Enhanced RNA or gene-specific pre-amplification protocols are available for projects with low DNA or RNA concentration.

PCR system design. If the gene of interest has been sequenced the facility can provide custom primer design through Primer Express 3 for research projects. Inventoried primers can also be purchased directly from Applied Biosystems.

Assays. More than 3,000 Real-time PCR systems are currently available in the facility including but not limited to: Apple, Chicken, Citrus, Dog, Horse, Cat, Human, Mouse, non-human Primate, Rat, Sturgeon, Tobacco and Trout. Assays can also be purchased. Please check our assays page for availability.

PCR. Complete PCR analysis is done using a laser 7900 HT platform. RNA and DNA 384-well bar-coded plates may be submitted from other labs. Plates from external users will NOT be accepted on Fridays or a day before a university holiday.

Final calculations. Results can be reported either by raw Cq values, using absolute standard methods or the comparative Cq method for relative gene transcription.

Custom design assays. The facility offers full disclosure of primer and probe sequences for newly designed assays. The facility can also provide a written Materials & Methods section for publications for preliminary data, microdissection and pre-amplification.

SNP. SNP genotyping analysis identifies single nucleotide polymorphisms, which are responsible for differences in genetic traits, susceptibility to disease or response to drug therapies. Over 4.5 million human and 10 thousand mouse SNP ready to order assays are available from Applied Biosystems.

 

 
Recharge Rates (internal clients paying with recharge # or DaFIS account #)
External Rates (external, non-Univ. of CA clients paying by check or credit card)
Processing samples
n=0-95
96-960
960+
n=0-95
96-960
960+
BioSprint RNA extraction (with cDNA synthesis) $16.00 $13.00 $29.00 $22.00
BioSprint RNA/DNA extraction (no cDNA synthesis) $12.00 $10.00   $21.00 $16.00  
Corbett RNA extraction (with cDNA synthesis) $23.00 $20.00   $41.00 $33.00  
Corbett RNA/DNA extraction (no cDNA synthesis) $16.00 $14.00   $29.00 $23.00  
Qiagen nucleic acid extraction - available for soil, fecal, paraffin - (with cDNA synthesis) $31.00     $64.00    
Qiagen nucleic acid extraction - available for soil, fecal, paraffin - (no cDNA synthesis) $26.00     $53.00    
cDNA synthesis only (when RNA submitted) $11.00 $9.00 please enquire $19.00 $14.00 please enquire

 

 
Recharge Rates (internal clients paying with recharge # or DaFIS account #)
External Rates (external, non-Univ. of CA clients paying by check or credit card)
qPCR Reaction
n=1-200
201-767
768-2499
n=1-200
201-767
768-2499
qPCR reaction - 12ul probe based $15.00 $14.00 $26.00 $25.00
qPCR reaction - 5ul probe based LDA     $6.00     $11.00
qPCR reaction - 12ul SYBR $16.00 $13.00   $27.00 $25.00  
qPCR reaction - 12ul multiplexing $25.00 $22.00   $45.00 $36.00  
qPCR reaction - 5ul SNP Genotyping $12.00 $10.00   $22.00 $17.00  

 

 
Recharge Rates (internal clients paying with recharge # or DaFIS account #)
External Rates (external, non-Univ. of CA clients paying by check or credit card)
Design and Validation
per assay
per assay
Design and validation - Roche UPL $144.00 $255.00
Design and validation - MWG probe $200.00 $295.00
Design and validation - LifeTech probe $239.00 $342.00
Design and validation - SYBR Green $122.00 $217.00
Assay validation only - probe based $67.00 $117.00
Assay validation only - SYBR Green $68.00 $121.00

 

 
Recharge Rates (internal clients paying with recharge # or DaFIS account #)
External Rates (external, non-Univ. of CA clients paying by check or credit card)
Misc. Services
Pre-amplification (under 50 genes) $33.00 $58.00
Pre-amplification (over 50 genes) $57.00 $101.00
Agilent bioanalysis: Per chip (NanoChip = 1-12 samples) $80.00 $143.00
Sequencing $158.00 $280.00

 

 
Recharge Rates (internal clients paying with recharge # or DaFIS account #)
External Rates (external, non-Univ. of CA clients paying by check or credit card)
Consumables
LifeTech inventoried Gene Expression Assay (250ul volume) $121.00 $173.00
LifeTech non-inventoried Gene Expression Assay $166.00 $236.00
Roche UPL, entire tube $195.00 $277.00
Roche UPL probe, 10ul aliquot $13.00 $19.00
MGB (LifeTech probe) $162.00 $231.00
MWG Probe $135.00 $192.00
MWG Primer $5.00 $8.00
TaqMan PCR Master Mix by LifeTech (5mL bottle) $260.00 $370.00
Optical Adhesives $2.00 $4.00
384-well bar coded PCR plates $7.00 $10.00
PCR run on thermocycler (client brings pre-pipetted 384-well plate to run) $22.00 $37.00
Primer/probe mix per reaction (sold in 5ul aliquots) $2.00 $4.00

 

 
Recharge Rates (internal clients paying with recharge # or DaFIS account #)
External Rates (external, non-Univ. of CA clients paying by check or credit card)
Consultation
per hour
per hour
Director $124.00 $176.00
Staff Research Associate II $39.00 $64.00
Staff Research Associate III $52.00 $84.00