1995, B.Sc., Wageningen University, Wageningen, The Netherlands
1997, M.Sc., Wageningen University, Wageningen, The Netherlands
2002, Ph.D., Wageningen University, Wageningen, The Netherlands
Active Research Grants
Principal Investigator, Toxoplasma proteins that modulate the host cell, (Principal Investigator), National Institutes of Health
Principal Investigator, Characterization of Sarcocystis neurona secreted proteins that could function as vaccine candidates to protect horses against the neurological disease Equine protozoal myeloencephalitis , (Principal Investigator), Center for Equine Health
Principal Investigator, Discovering essential Toxoplasma Sporozoite Genes: Production of uninfective oocysts, (Principal Investigator), Center for Companion Animal Health
Trainer, Modulation of the human immune response by the heart parasite Toxoplasma gondii, Debanjan Mukhopadhyay (Principal Investigator), American Heart Association
Trainer, Identification and characterization of Toxoplasma genes that determine fitness in interferon gamma-stimulated human cells, Shruthi Krishnamurthi (Principal Investigator), American Heart Association
Principal Investigator, Regulatory factors that determine Toxoplasma bradyzoite to merozoites conversion , (Principal Investigator), National Institutes of Health
Principal Investigator, Genome-wide CRISPR/Cas9-mediated loss-of-function screens to identify Toxoplasma genes that determine fitness in gamma interferon-stimulated human cells, (Principal Investigator), NIH -PENDING -
Principal Investigator, Discovering essential toxoplasma sporozoite genes: production of uninfective oocysts, (Principal Investigator), CCAH
Principal Investigator, Identify Toxoplasma genes that determine in vivo fitness with CRISRP-Cas9 genetic screens, (Principal Investigator), NIH
Principal Investigator, Identification of the mechanism by which Toxoplasma activates the NLRP1 inflammasome , (Principal Investigator), NIH
Principal Investigator, Synthetic lethality screens in a nutrient sensitized Toxoplasma strain to identify novel proteins that mediate nutrient acquisition in chronic Toxoplasma infection, (Principal Investigator), NIH
Co-Principal Investigator, Bridging international expertise to address Toxoplasma transmission in a changing climate, Karen Shapiro (Principal Investigator), UC Davis
Most Recent Five Book Chapters
2020 Mukhopadhyay D, Saeij JPJ : Assays to evaluate Toxoplasma-macrophage interactions. In: Toxoplasma gondii: Methods and protocols, Tonkin C, (ed), , . 347-370.
2002 Verburg-van Kemenade BML, Engelsma MY, Huising MO, Kwang J, van Muiswinkel WB, Saeij JP, Metz JR, Flik G : Crosstalk between the neuro-endocrine and immune system in teleosts, Goos HJ Th [and others], (ed), Perspective in Comparative Endocrinology: Unity and Diversity, Bologna, Italy. 359-67.
Molecular basis of pathogenesis of Apicomplexan parasites in humans and livestock. Apicomplexan parasites are a major cause of mortality and morbidity in humans and livestock. These intracellular parasites secrete proteins that manipulate host cells to create the niche in which they can replicate. In turn, host cells have intricate mechanisms to detect and combat these parasites. As a consequence these parasites and their hosts co-evolve, leading to variation in both host and parasite genomes and to the emergence of differences in parasite virulence and host resistance. I am interested in how the interactions between parasite and host can lead to disease with a special interest in the genetic basis for individual host differences in resistance and parasite differences in virulence. A major effort of my lab is to identify genes from a model apicomplexan parasite, Toxoplasma gondii, that mediate the modulation of the host cell and to characterize the proteins encoded by these Toxoplasma genes, their host cell interaction partners and their role in virulence and co-option of the host cell. We also study host genetic factors involved in resistance to Toxoplasma. We try and apply what we have learned from our work with Toxoplasma to veterinary diseases caused by other apicomplexan parasites such as Neospora spp. and Sarcocystis neurona.
Genetics, Genomics, Parasitology, Immunology, Biochemistry, Bioinformatics, Molecular Biology, Human Disease, Animal Disease.